Gene Delivery to Mammalian Cells(Methods in Molecular Biology Series): Volume 1: Nonviral Gene Transfer Techniques

Twenty-four papers describe protocols for gene delivery using a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, and hydrodynamics. Each protocol includes step-by-step instructions, background notes, lists of equipment and reagents, and troubleshooting advice. Introductory chapters review the delivery methods, discuss their advantages and limitations, describe their successful uses, and examine future prospects for the technology.

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