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Genetic Engineering with PCR


Autoři: Editors: Robert M. Horton and Robert C. Tait
Vydavatelství: Horizon Scientific Press
While there have been myriad books written on site-directed mutagenesis, and portions of several PCR books address the topic, this is the first laboratory manual that focuses on synthetic (as opposed to analytical) uses of PCR. PCR-mediated sequence manipulation gives the genetic engineer such precise control over construction, mutation, and recombination of sequences that it represents a new generation of DNA technology. There are many applications, innovations, and refinements of the use of PCR for synthetic purposes. This entire volume is devoted to cover the topic in breadth and depth and includes a variety of approaches for mutagenesis, recombination, and the construction of synthetic or semi-synthetic genes.



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