EMBO Practical Course: "Lipid rafts: methods for studying membrane organization"
Dresden, Germany (May 23-31, 2010)
The practical course will be concerned with the following topics:
•
The use and pitfalls of detergent extraction•
The preparation of plasma membrane vesicle and spheres for phase separation studies•
Giant unilamelar vesicles of model membranes•
Lipid protein interactions using reconstituted liposomes and giant unilamelar vesicles•
Measurement of membrane order employing c-laurdan•
Lipid analysis by mass spectrometry•
Florescence correlation- and STED microscopy•
Fluorescent lipids for cell biology
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